Format du dépôt |
Fichier |
Type de dépôt |
Article dans une revue |
Titre |
en
The Simple One-Step (SOS) Stool Processing Method for Use with the Xpert MTB/RIF Assay for a Child-Friendly Diagnosis of Tuberculosis Closer to the Point of Care
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Résumé |
en
Young children cannot easily produce sputum for diagnosis of pulmonary tuberculosis (TB). Alternatively, Mycobacterium tuberculosis complex bacilli can be detected in stool by using the Xpert MTB/RIF (Ultra) assay (Xpert). Published stool processing methods contain somewhat complex procedures and require additional supplies. The aim of this study was to develop a simple one-step (SOS) stool processing method based on gravity sedimentation only, similar to Xpert testing of sputum samples, for the detection of M. tuberculosis in stool samples. We first assessed whether the SOS stool method could provide valid Xpert results without the need for bead-beating, dilution, and filtration steps. We concluded that this was the case, and we then validated the SOS stool method by testing spiked stool samples. By using the SOS stool method, 27 of the 29 spiked samples gave valid Xpert results, and M. tuberculosis was recovered from all 27 samples. The proof of principle of the SOS stool method was demonstrated in routine settings in Addis Ababa, Ethiopia. Nine of 123 children with presumptive TB had M. tuberculosis-positive results for nasogastric aspiration (NGA) samples, and 7 (77.8%) of those children also had M. tuberculosis-positive Xpert results for stool samples. Additionally, M. tuberculosis was detected in the stool samples but not the NGA samples from 2 children. The SOS stool processing method makes use of the standard Xpert assay kit, without the need for additional supplies or equipment. The method can potentially be rolled out to any Xpert site, bringing a bacteriologically confirmed diagnosis of TB in children closer to the point of care.
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Auteur(s)
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Petra de Haas
, Bazezew Yenew
1
, Endale Mengesha
, Andrii Slyzkyi
, Zewdu Gashu
, Manon Lounnas
2, 3
, Ephrem Tesfaye
1
, Ahmed Bedru
, Edine Tiemersma
, Kristin Kremer
, Misikir Amare
1
, Getu Diriba
1
, Betselot Zerihun
1
, Tilaye Gudina
, Ben Tegegn
, Maryline Bonnet
4
, Challa Negeri
, Eveline Klinkenberg
, Kristin Kremer
5
1
EPHI -
Ethiopian Public Health Institute
( 525050 )
- Addis Ababa
- Éthiopie
2
CHRU Montpellier -
Centre Hospitalier Régional Universitaire [Montpellier]
( 258773 )
- 191 avenue du doyen Gaston Giraud 34295 Montpellier
- France
3
MIVEGEC -
Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle
( 166573 )
- 911 Avenue Agropolis BP 64501 34394 Montpellier cedex 5
- France
-
Université de Montpellier UMR5290 ( 410122 )
;
-
Centre National de la Recherche Scientifique UMR5290 ( 441569 )
;
-
Institut de Recherche pour le Développement 224 ( 451860 )
4
TransVIHMI -
Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes
( 1087128 )
- Centre IRD France Sud 911, avenue Agropolis BP 64501 F-34394 Montpellier cedex 5
- France
-
Institut de Recherche pour le Développement UMI 233 ( 67872 )
;
-
Université de Yaoundé I ( 217395 )
;
-
Institut National de la Santé et de la Recherche Médicale U 1175 ( 303623 )
;
-
Université de Montpellier ( 410122 )
;
-
Université Cheikh Anta Diop de Dakar [Sénégal] ( 1175215 )
5
Amsterdam UMC - Amsterdam University Medical Center
( 565705 )
- Amsterdam
- Pays-Bas
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Langue du document |
Anglais
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Licence |
Paternité
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Nom de la revue |
|
Date de publication |
2021-07-19
|
Volume |
59
|
Numéro |
8
|
Page/Identifiant |
e0040621
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Domaine(s) |
-
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie
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Mots-clés (Mesh) |
-
Child, Preschool
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Ethiopia
-
Humans
-
Mycobacterium tuberculosis
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Point-of-Care Systems
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Sensitivity and Specificity
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Sputum
-
Tuberculosis
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Mots-clés |
en
Ethiopia, Xpert MTB/RIF, Bacteriologically confirmed, Children, Diagnosis, Point of care, Simple one-step stool method, Sputum, Stool, Tuberculosis
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DOI |
10.1128/jcm.00406-21 |
Base Horizon |
fdi:010083293 |
Pubmed Id |
34076469 |
PubMed Central |
PMC8373220 |
UT key WOS |
000708274700010 |