Identification of microbial communities involved in the methane cycle of a freshwater meromictic lake.
Corinne Biderre-Petit
(1)
,
Didier Jézéquel
(2, 3)
,
Eric Dugat-Bony
(1)
,
Filipa Lopes
(3)
,
Jan Kuever
(4)
,
Guillaume Borrel
(1)
,
Eirc Viollier
(3)
,
Gérard Fonty
(1)
,
Pierre Peyret
(5)
Corinne Biderre-Petit
- Fonction : Auteur
- PersonId : 176556
- IdHAL : corinne-petit
- IdRef : 083628975
Eric Dugat-Bony
- Fonction : Auteur
- PersonId : 735047
- IdHAL : eric-dugat-bony
- ORCID : 0000-0002-5182-0063
- IdRef : 161970990
Filipa Lopes
- Fonction : Auteur
- PersonId : 764124
- ORCID : 0000-0002-0156-0048
- IdRef : 158150430
Guillaume Borrel
- Fonction : Auteur
- PersonId : 746592
- IdHAL : guillaumeborrelpasteurfr
- ORCID : 0000-0003-4893-8180
- IdRef : 175403406
Pierre Peyret
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- Fonction : Auteur correspondant
- PersonId : 867738
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Résumé
Lake Pavin is a meromictic crater lake located in the French Massif Central area. In this ecosystem, most methane (CH(4)) produced in high quantity in the anoxic bottom layers, and especially in sediments, is consumed in the water column, with only a small fraction of annual production reaching the atmosphere. This study assessed the diversity of methanogenic and methanotrophic populations along the water column and in sediments using PCR and reverse transcription-PCR-based approaches targeting functional genes, i.e. pmoA (α-subunit of the particulate methane monooxygenase) for methanotrophy and mcrA (α-subunit of the methyl-coenzyme M reductase) for methanogenesis as well as the phylogenetic 16S rRNA genes. Although methanogenesis rates were much higher in sediments, our results confirm that CH(4) production also occurs in the water column where methanogens were almost exclusively composed of hydrogenotrophic methanogens, whereas both hydrogenotrophs and acetotrophs were almost equivalent in the sediments. Sequence analysis of markers, pmoA and the 16S rRNA gene, suggested that Methylobacter may be an important group actively involved in CH(4) oxidation in the water column. Two main phylotypes were characterized, one of which could consume CH(4) under conditions where the oxygen amount is undetectable.
Domaines
Biodiversité et EcologieFormat du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Identification of microbial communities involved in the methane cycle of a freshwater meromictic lake.
|
Résumé |
en
Lake Pavin is a meromictic crater lake located in the French Massif Central area. In this ecosystem, most methane (CH(4)) produced in high quantity in the anoxic bottom layers, and especially in sediments, is consumed in the water column, with only a small fraction of annual production reaching the atmosphere. This study assessed the diversity of methanogenic and methanotrophic populations along the water column and in sediments using PCR and reverse transcription-PCR-based approaches targeting functional genes, i.e. pmoA (α-subunit of the particulate methane monooxygenase) for methanotrophy and mcrA (α-subunit of the methyl-coenzyme M reductase) for methanogenesis as well as the phylogenetic 16S rRNA genes. Although methanogenesis rates were much higher in sediments, our results confirm that CH(4) production also occurs in the water column where methanogens were almost exclusively composed of hydrogenotrophic methanogens, whereas both hydrogenotrophs and acetotrophs were almost equivalent in the sediments. Sequence analysis of markers, pmoA and the 16S rRNA gene, suggested that Methylobacter may be an important group actively involved in CH(4) oxidation in the water column. Two main phylotypes were characterized, one of which could consume CH(4) under conditions where the oxygen amount is undetectable.
|
Auteur(s) |
Corinne Biderre-Petit
1
, Didier Jézéquel
2, 3
, Eric Dugat-Bony
1
, Filipa Lopes
3
, Jan Kuever
4
, Guillaume Borrel
1
, Eirc Viollier
3
, Gérard Fonty
1
, Pierre Peyret
5
1
LMGE -
Laboratoire Microorganismes : Génome et Environnement
( 110077 )
- Campus Universitaire des Cézeaux, TSA 60026, 1 Impasse Amélie Murat, 63178 Aubière
- France
2
IPGP -
Institut de Physique du Globe de Paris
( 1852 )
- IPGP, 1 rue Jussieu, 75238 Paris cedex 05 ; Université Paris Diderot, Bât. Lamarck A case postale 7011, 75205 Paris CEDEX 13
- France
3
LGE -
Laboratoire de géochimie des Eaux
( 43029 )
- Université Paris Diderot - case 7052 - 35 Rue Hélène Brion - 75205 Paris Cedex 13 France / IPGP, Boîte 89 - 1, rue Cuvier - 75252 Paris Cedex 05 - France
- France
4
IWT -
Institute of Materials Engineering [Bremen]
( 558060 )
- Badgasteiner Str. 3 28359 Bremen
- Allemagne
5
CIDAM -
Conception, Ingénierie et Développement de l'Aliment et du Médicament
( 201821 )
- Facultés de Médecine et de Pharmacie CBRV / RdC et 5ème étage BP 38 / 28, Place Henri-Dunant 63001 / Clermont-Ferrand
- France
|
Page/Identifiant |
533-45
|
Comité de lecture |
Oui
|
Vulgarisation |
Non
|
Langue du document |
Anglais
|
Nom de la revue |
|
Audience |
Internationale
|
Date de publication |
2011-09
|
Volume |
77
|
Numéro |
3
|
Public visé |
Scientifique
|
Sous-type de document pour les Articles |
Research article
|
Domaine(s) |
|
Financement |
|
Mots-clés |
sq
Lake Pavin, Methane
|
DOI | 10.1111/j.1574-6941.2011.01134.x |
ProdINRA | 355223 |
Pubmed Id | 21595728 |
UT key WOS | 000293684700006 |
Fichier principal
Biderre-Petit2011_Identification of microbial communities involved in the methane cycle of a freshwater meromictic lake_1.pdf ( 455.82 Ko
)
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