Venomics survey of six myrmicine ants provides insights into the molecular and structural diversity of their peptide toxins
Valentine Barassé
(1)
,
Nathan Téné
(1)
,
Christophe Klopp
(2)
,
Françoise Paquet
(3)
,
Niklas Tysklind
(4)
,
Valérie Troispoux
(4)
,
Hadrien Lalägue
(4)
,
Jérôme Orivel
(4)
,
Benjamin Lefranc
(5)
,
Jérôme Leprince
(5)
,
Martin Kenne
(6)
,
Maurice Tindo
(6)
,
Michel Treilhou
(1)
,
Axel Touchard
(1)
,
Elsa Bonnafé
(1)
1
BTSB -
Biochimie et Toxicologie des Substances Bioactives
2 MIAT INRAE - Unité de Mathématiques et Informatique Appliquées de Toulouse
3 CBM - Centre de biophysique moléculaire
4 UMR ECOFOG - Ecologie des forêts de Guyane
5 PRIMACEN - Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie
6 Université de Douala
2 MIAT INRAE - Unité de Mathématiques et Informatique Appliquées de Toulouse
3 CBM - Centre de biophysique moléculaire
4 UMR ECOFOG - Ecologie des forêts de Guyane
5 PRIMACEN - Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie
6 Université de Douala
Valentine Barassé
- Fonction : Auteur
- PersonId : 744202
- IdHAL : valentine-barasse
- ORCID : 0000-0001-8021-2086
- IdRef : 252779614
Nathan Téné
- Fonction : Auteur
- PersonId : 744203
- IdHAL : joseph-nathan-tene-ghomsi
- ORCID : 0000-0003-3958-8182
Christophe Klopp
- Fonction : Auteur
- PersonId : 735911
- IdHAL : 0000-0001-7126-5477
- ORCID : 0000-0001-7126-5477
Françoise Paquet
- Fonction : Auteur
- PersonId : 174884
- IdHAL : francoise-paquet
- ORCID : 0000-0001-8838-3445
- IdRef : 080021751
Niklas Tysklind
- Fonction : Auteur
- PersonId : 736848
- IdHAL : niklas-tysklind
- ORCID : 0000-0002-6617-7875
- IdRef : 201735032
Jérôme Orivel
- Fonction : Auteur
- PersonId : 739128
- IdHAL : jerome-orivel
- ORCID : 0000-0002-3710-5354
- IdRef : 052459438
Benjamin Lefranc
- Fonction : Auteur
- PersonId : 1349193
Jérôme Leprince
- Fonction : Auteur
- PersonId : 170122
- IdHAL : jerome-leprince
- ORCID : 0000-0002-7814-9927
- IdRef : 159285593
Michel Treilhou
- Fonction : Auteur
- PersonId : 184162
- IdHAL : michel-treilhou
- ORCID : 0000-0002-3364-1950
- IdRef : 109473434
Axel Touchard
- Fonction : Auteur
- PersonId : 1158978
- ORCID : 0000-0002-7766-0088
Elsa Bonnafé
- Fonction : Auteur
- PersonId : 743963
- IdHAL : elsa-bonnafe
- ORCID : 0000-0002-2630-4876
- IdRef : 082114889
Résumé
Among ants, Myrmicinae represents the most speciose subfamily. The venom composition previously described for these social insects is extremely variable, with alkaloids predominant in some genera while, conversely, proteomics studies have revealed that some myrmicine ant venoms are peptide-rich. Using integrated transcriptomic and proteomic approaches, we characterized the venom peptidomes of six ants belonging to the different tribes of Myrmicinae. We identified a total of 79 myrmicitoxins precursors which can be classified into 38 peptide families according to their mature sequences. Myrmicine ant venom peptidomes showed heterogeneous compositions, with linear and disulfide-bonded monomers as well as dimeric toxins. Several peptide families were exclusive to a single venom whereas some were retrieved in multiple species. A hierarchical clustering analysis of precursor signal sequences led us to divide the myrmicitoxins precursors into eight families, including some that have already been described in other aculeate hymenoptera such as secapin-like peptides and voltage-gated sodium channel (Na V) toxins. Evolutionary and structural analyses of two representatives of these families highlighted variation and conserved patterns that might be crucial to explain myrmicine venom peptide functional adaptations to biological targets. Key contribution This study provides novel insights into both peptide diversity and diversification of myrmicine ant venoms.
Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Venomics survey of six myrmicine ants provides insights into the molecular and structural diversity of their peptide toxins
|
Résumé |
en
Among ants, Myrmicinae represents the most speciose subfamily. The venom composition previously described for these social insects is extremely variable, with alkaloids predominant in some genera while, conversely, proteomics studies have revealed that some myrmicine ant venoms are peptide-rich. Using integrated transcriptomic and proteomic approaches, we characterized the venom peptidomes of six ants belonging to the different tribes of Myrmicinae. We identified a total of 79 myrmicitoxins precursors which can be classified into 38 peptide families according to their mature sequences. Myrmicine ant venom peptidomes showed heterogeneous compositions, with linear and disulfide-bonded monomers as well as dimeric toxins. Several peptide families were exclusive to a single venom whereas some were retrieved in multiple species. A hierarchical clustering analysis of precursor signal sequences led us to divide the myrmicitoxins precursors into eight families, including some that have already been described in other aculeate hymenoptera such as secapin-like peptides and voltage-gated sodium channel (Na V) toxins. Evolutionary and structural analyses of two representatives of these families highlighted variation and conserved patterns that might be crucial to explain myrmicine venom peptide functional adaptations to biological targets. Key contribution This study provides novel insights into both peptide diversity and diversification of myrmicine ant venoms.
|
Auteur(s) |
Valentine Barassé
1
, Nathan Téné
1
, Christophe Klopp
2
, Françoise Paquet
3
, Niklas Tysklind
4
, Valérie Troispoux
4
, Hadrien Lalägue
4
, Jérôme Orivel
4
, Benjamin Lefranc
5
, Jérôme Leprince
5
, Martin Kenne
6
, Maurice Tindo
6
, Michel Treilhou
1
, Axel Touchard
1
, Elsa Bonnafé
1
1
BTSB -
Biochimie et Toxicologie des Substances Bioactives
( 483384 )
- INU Champollion
Place de Verdun - 81000 Albi
- France
2
MIAT INRAE -
Unité de Mathématiques et Informatique Appliquées de Toulouse
( 1002385 )
- Chemin de Borde Rouge, 31320 Castanet Tolosan
- France
3
CBM -
Centre de biophysique moléculaire
( 451 )
- Rue Charles Sadron 45071 ORLEANS CEDEX 2
- France
4
UMR ECOFOG -
Ecologie des forêts de Guyane
( 1002453 )
- 745 BP 709 - F-97387 Kourou Cedex France
- Guyane française
5
PRIMACEN -
Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie
( 224876 )
- Faculté des Sciences et Techniques Place Emile Blondel 76821 Mont-Saint-Aignan Cedex
- France
6
Université de Douala
( 217369 )
- B.P. 2701 Douala
- Cameroun
|
Nom de la revue |
|
Langue du document |
Anglais
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Volume |
151
|
Date de publication |
2022
|
Licence |
Copyright (Tous droits réservés)
|
Public visé |
Scientifique
|
Page/Identifiant |
pp. 103876
|
Référence interne |
|
Domaine(s) |
|
Mots-clés |
en
Toxin precursor, Secapins, Voltage-gated sodium channel (Na V ) peptide, NMR structure, Dimeric peptide
|
DOI | 10.1016/j.ibmb.2022.103876 |
Pubmed Id | 36410579 |
Origine :
Fichiers produits par l'(les) auteur(s)
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