Cytotoxicity and Identification of Antibacterial Compounds from Baillonella toxisperma Bark Using a LC-MS/MS and Molecular Networking Approach
Morel Essono Mintsa
(1)
,
Brice Serge Kumulungui
,
Cédric Sima Obiang
,
Elodie Dussert
(2, 3)
,
Elodie Choque
(1, 4)
,
Damien Herfurth
(1, 5)
,
Rozenn Ravallec
(2, 6, 7, 8, 3)
,
Joseph-Privat Ondo
(9)
,
François Mesnard
(1, 4)
1
BioEcoAgro BIOPI-UPJV
2 BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
3 BioEcoAgro - Equipe 7 - Health benefit of protein hydrolysates and agro-food co-products: control of production, characterization and valorisation
4 BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
5 BioEcoAgro - Equipe 1 - Functioning and adaptation of the plant in interaction with its environment
6 ICV - Institut Charles Viollette (ICV) - EA 7394
7 ProBioGEM - Procédés Biologiques, Génie Enzymatique et Microbien - EA1026
8 LTSN - Laboratoire de Technologie des Substances Naturelles
9 USTM - Université des Sciences et Techniques de Masuku
2 BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
3 BioEcoAgro - Equipe 7 - Health benefit of protein hydrolysates and agro-food co-products: control of production, characterization and valorisation
4 BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
5 BioEcoAgro - Equipe 1 - Functioning and adaptation of the plant in interaction with its environment
6 ICV - Institut Charles Viollette (ICV) - EA 7394
7 ProBioGEM - Procédés Biologiques, Génie Enzymatique et Microbien - EA1026
8 LTSN - Laboratoire de Technologie des Substances Naturelles
9 USTM - Université des Sciences et Techniques de Masuku
Morel Essono Mintsa
- Fonction : Auteur
- PersonId : 1258513
- ORCID : 0000-0002-4654-7277
Brice Serge Kumulungui
- Fonction : Auteur
Cédric Sima Obiang
- Fonction : Auteur
Elodie Choque
- Fonction : Auteur
- PersonId : 1151835
- ORCID : 0000-0002-5261-0738
- IdRef : 16652882X
Rozenn Ravallec
- Fonction : Auteur
- PersonId : 761959
- IdHAL : rravallec
- ORCID : 0000-0002-8697-5225
- IdRef : 191322628
Résumé
Baillonella toxisperma is a medicinal plant used in northern Gabon to treat microbial diseases. It is a plant well-known by local populations, but very few studies have focused on the molecules responsible for the antibacterial activities of B. toxisperma. This study proposes a dereplication strategy based on molecular networking generated from HPLC-ESI-Q/TOF data, allowing investigation of the molecules responsible for the antibacterial activity of B. toxisperma. From this strategy, eighteen compounds were putatively identified. All of these compounds belonged mainly to five families of natural compounds, including phenylpropanolamines, stilbenes, flavonoids, lignans and phenolic glycosides. The chemical study carried out from the bark of B. toxisperma allowed us to identify, for the first time, compounds such as resveratrol and derivatives, epicatechin, epigallocatechin and epigallocatechin gallate. In addition, antibacterial activity (diffusion method and microdilution) and cytotoxicity (Cell Counting Kit-8 (CCK-8 Assay)) in vitro were evaluated. The crude ethanolic extract, as well as the fractions of B. toxisperma, showed significant antibacterial activity. However, the ethanolic fractions F2 and F4 presented high antibacterial activity compared to the crude extract. Cytotoxicity studies on colon-cancer cells (Caco-2) and human keratinocyte cells (HaCaT) showed moderate cytotoxicity in both cell types. This study clearly shows the therapeutic potential of the ethanolic extract of the bark of B. toxisperma and provides information on the phytochemical composition and bioactive compounds of the plant.
Domaines
Sciences du Vivant [q-bio]Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Cytotoxicity and Identification of Antibacterial Compounds from Baillonella toxisperma Bark Using a LC-MS/MS and Molecular Networking Approach
|
Résumé |
en
Baillonella toxisperma is a medicinal plant used in northern Gabon to treat microbial diseases. It is a plant well-known by local populations, but very few studies have focused on the molecules responsible for the antibacterial activities of B. toxisperma. This study proposes a dereplication strategy based on molecular networking generated from HPLC-ESI-Q/TOF data, allowing investigation of the molecules responsible for the antibacterial activity of B. toxisperma. From this strategy, eighteen compounds were putatively identified. All of these compounds belonged mainly to five families of natural compounds, including phenylpropanolamines, stilbenes, flavonoids, lignans and phenolic glycosides. The chemical study carried out from the bark of B. toxisperma allowed us to identify, for the first time, compounds such as resveratrol and derivatives, epicatechin, epigallocatechin and epigallocatechin gallate. In addition, antibacterial activity (diffusion method and microdilution) and cytotoxicity (Cell Counting Kit-8 (CCK-8 Assay)) in vitro were evaluated. The crude ethanolic extract, as well as the fractions of B. toxisperma, showed significant antibacterial activity. However, the ethanolic fractions F2 and F4 presented high antibacterial activity compared to the crude extract. Cytotoxicity studies on colon-cancer cells (Caco-2) and human keratinocyte cells (HaCaT) showed moderate cytotoxicity in both cell types. This study clearly shows the therapeutic potential of the ethanolic extract of the bark of B. toxisperma and provides information on the phytochemical composition and bioactive compounds of the plant.
|
Auteur(s) |
Morel Essono Mintsa
1
, Brice Serge Kumulungui
, Cédric Sima Obiang
, Elodie Dussert
2, 3
, Elodie Choque
1, 4
, Damien Herfurth
1, 5
, Rozenn Ravallec
2, 6, 7, 8, 3
, Joseph-Privat Ondo
9
, François Mesnard
1, 4
1
BioEcoAgro BIOPI-UPJV
( 1185900 )
- UFR des Sciences, Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens cedex
- France
2
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
( 1057473 )
- UMRT 1158 -
* Site de Laon - Pôle du Griffon - 180 rue Pierre-Gilles de Gennes - 02000 BARENTON-BUGNY
* Site de l'Ecole Polytechnique Universitaire de Lille -
Institut VIOLLETTE- Aile C, Avenue Paul Langevin, 59655 Villeneuve d'Ascq Cedex
- France
3
BioEcoAgro - Equipe 7 - Health benefit of protein hydrolysates and agro-food co-products: control of production, characterization and valorisation
( 1185505 )
- Site de Laon - Pôle du Griffon - 180 rue Pierre-Gilles de Gennes - 02000 BARENTON-BUGNY * Site de l'Ecole Polytechnique Universitaire de Lille - Institut VIOLLETTE- Aile C, Avenue Paul Langevin, 59655 Villeneuve d'Ascq Cedex
- France
4
BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
( 1185498 )
- Site de Laon - Pôle du Griffon - 180 rue Pierre-Gilles de Gennes - 02000 BARENTON-BUGNY * Site de l'Ecole Polytechnique Universitaire de Lille - Institut VIOLLETTE- Aile C, Avenue Paul Langevin, 59655 Villeneuve d'Ascq Cedex
- France
5
BioEcoAgro - Equipe 1 - Functioning and adaptation of the plant in interaction with its environment
( 1185497 )
- Site de Laon - Pôle du Griffon - 180 rue Pierre-Gilles de Gennes - 02000 BARENTON-BUGNY * Site de l'Ecole Polytechnique Universitaire de Lille - Institut VIOLLETTE- Aile C, Avenue Paul Langevin, 59655 Villeneuve d'Ascq Cedex
- France
6
ICV -
Institut Charles Viollette (ICV) - EA 7394
( 423294 )
- Polytech'Lille
Avenue Paul Langevin - Cité Scientifique
59655 Villeneuve d'Ascq - France
- France
7
ProBioGEM -
Procédés Biologiques, Génie Enzymatique et Microbien - EA1026
( 183821 )
- UPRES , IUT A-Polytech'Lille, USTL, Lille
Fusion en 2015 vers ICV EA 7394
- France
8
LTSN -
Laboratoire de Technologie des Substances Naturelles
( 58508 )
- USTL - Villeneuve d'Ascq
(A l'origine de ProBioGEM)
- France
9
USTM -
Université des Sciences et Techniques de Masuku
( 377466 )
- Franceville
- Gabon
|
Public visé |
Scientifique
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Langue du document |
Anglais
|
Nom de la revue |
|
Date de publication |
2023-04-27
|
Volume |
13
|
Numéro |
5
|
Page/Identifiant |
599
|
Domaine(s) |
|
Mots-clés |
en
Baillonella toxisperma, antibacterial activity, cytotoxicity, dereplication, molecular networks
|
DOI | 10.3390/metabo13050599 |
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