Leishmania infections: Molecular targets and diagnosis
Mohammad Akhoundi
(1, 2)
,
Tim Downing
(3)
,
Jan Votýpka
(4, 5)
,
Katrin Kuhls
,
Julius Lukeš
,
Arnaud Cannet
(6)
,
Christophe Ravel
(7)
,
Pierre Marty
(1, 6)
,
Pascal Delaunay
(1, 6, 2)
,
Mohamed Kasbari
(8)
,
Bruno Granouillac
(9, 10)
,
Luigi Gradoni
(11)
,
Denis Sereno
(2, 12)
1
Laboratoire de Parasitologie-Mycologie, Nice
2 MIVEGEC - Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle
3 DCU - Dublin City University [Dublin]
4 Institute of Parasitology
5 UK - Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
6 C3M - Centre méditerranéen de médecine moléculaire
7 CNR-L - Centre National de Référence des Leishmanioses [CHRU Montpellier]
8 ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
9 TransVIHMI - Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes
10 MIVEGEC-DEEVA - Diversity, ecology, evolution & Adaptation of arthropod vectors
11 ISS - Istituto Superiore di Sanità = National Institute of Health
12 UMR INTERTRYP - Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides
2 MIVEGEC - Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle
3 DCU - Dublin City University [Dublin]
4 Institute of Parasitology
5 UK - Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
6 C3M - Centre méditerranéen de médecine moléculaire
7 CNR-L - Centre National de Référence des Leishmanioses [CHRU Montpellier]
8 ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
9 TransVIHMI - Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes
10 MIVEGEC-DEEVA - Diversity, ecology, evolution & Adaptation of arthropod vectors
11 ISS - Istituto Superiore di Sanità = National Institute of Health
12 UMR INTERTRYP - Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides
Mohammad Akhoundi
- Fonction : Auteur
- PersonId : 1031701
Katrin Kuhls
- Fonction : Auteur
Julius Lukeš
- Fonction : Auteur
Christophe Ravel
- Fonction : Auteur
- PersonId : 173725
- IdHAL : christophe-ravel
- ORCID : 0000-0002-7590-582X
- IdRef : 11799393X
Bruno Granouillac
- Fonction : Auteur
- PersonId : 738171
- IdHAL : bruno-granouillac
- ORCID : 0009-0003-8869-1247
- IdRef : 253135966
Luigi Gradoni
- Fonction : Auteur
- PersonId : 1007005
Résumé
Progress in the diagnosis of leishmaniases depends on the development of effective methods and the discovery of suitable biomarkers. We propose firstly an update classification of Leishmania species and their synonymies. We demonstrate a global map highlighting the geography of known endemic Leishmania species pathogenic to humans. We summarize a complete list of techniques currently in use and discuss their advantages and limitations. The available data highlights the benefits of molecular markers in terms of their sensitivity and specificity to quantify variation from the subgeneric level to species complexes, (sub) species within complexes, and individual populations and infection foci. Each DNA-based detection method is supplied with a comprehensive description of markers and primers and proposal for a classification based on the role of each target and primer in the detection, identification and quantification of leishmaniasis infection. We outline a genome-wide map of genes informative for diagnosis that have been used for Leishmania genotyping. Furthermore, we propose a classification method based on the suitability of well-studied molecular markers for typing the 21 known Leishmania species pathogenic to humans. This can be applied to newly discovered species and to hybrid strains originating from inter-species crosses. Developing more effective and sensitive diagnostic methods and biomarkers is vital for enhancing Leishmania infection control programs.
Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Leishmania infections: Molecular targets and diagnosis
|
Résumé |
en
Progress in the diagnosis of leishmaniases depends on the development of effective methods and the discovery of suitable biomarkers. We propose firstly an update classification of Leishmania species and their synonymies. We demonstrate a global map highlighting the geography of known endemic Leishmania species pathogenic to humans. We summarize a complete list of techniques currently in use and discuss their advantages and limitations. The available data highlights the benefits of molecular markers in terms of their sensitivity and specificity to quantify variation from the subgeneric level to species complexes, (sub) species within complexes, and individual populations and infection foci. Each DNA-based detection method is supplied with a comprehensive description of markers and primers and proposal for a classification based on the role of each target and primer in the detection, identification and quantification of leishmaniasis infection. We outline a genome-wide map of genes informative for diagnosis that have been used for Leishmania genotyping. Furthermore, we propose a classification method based on the suitability of well-studied molecular markers for typing the 21 known Leishmania species pathogenic to humans. This can be applied to newly discovered species and to hybrid strains originating from inter-species crosses. Developing more effective and sensitive diagnostic methods and biomarkers is vital for enhancing Leishmania infection control programs.
|
Auteur(s) |
Mohammad Akhoundi
1, 2
, Tim Downing
3
, Jan Votýpka
4, 5
, Katrin Kuhls
, Julius Lukeš
, Arnaud Cannet
6
, Christophe Ravel
7
, Pierre Marty
1, 6
, Pascal Delaunay
1, 6, 2
, Mohamed Kasbari
8
, Bruno Granouillac
9, 10
, Luigi Gradoni
11
, Denis Sereno
2, 12
1
Laboratoire de Parasitologie-Mycologie, Nice
( 187497 )
- CHU L'Archet-II, Nice
- France
2
MIVEGEC -
Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle
( 166573 )
- 911 Avenue Agropolis BP 64501 34394 Montpellier cedex 5
- France
3
DCU -
Dublin City University [Dublin]
( 300829 )
- Glanevin, Dublin 9
- Irlande
4
Institute of Parasitology
( 21630 )
- Branisovska 31 370 05 Ceske Budejovice
- Tchéquie
5
UK -
Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
( 304738 )
- Ovocný trh 5
Prague 1
116 36
Czech Republic
- Tchéquie
6
C3M -
Centre méditerranéen de médecine moléculaire
( 180122 )
- Hôpital de l'Archet, 151 rte Saint Antoine de Ginestiere 06204 Nice Cedex 3
- France
7
CNR-L -
Centre National de Référence des Leishmanioses [CHRU Montpellier]
( 507252 )
- Département de Parasitologie-Mycologie
39, avenue Charles Flahault
34 295 MONTPELLIER CEDEX 5
- France
8
ANSES -
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
( 301715 )
- 14 rue Pierre et Marie Curie
94701 MAISONS-ALFORT Cedex
- France
9
TransVIHMI -
Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes
( 1087128 )
- Centre IRD France Sud 911, avenue Agropolis BP 64501 F-34394 Montpellier cedex 5
- France
10
MIVEGEC-DEEVA -
Diversity, ecology, evolution & Adaptation of arthropod vectors
( 1052755 )
- France
11
ISS -
Istituto Superiore di Sanità = National Institute of Health
( 359183 )
- 299, Viale Regina Elena, 00161 – Roma
- Italie
12
UMR INTERTRYP -
Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides
( 131738 )
- Campus International de Baillarguet TA A-17/G 34398 Montpellier cedex 5
- France
|
Licence |
Paternité - Pas d'utilisation commerciale - Pas de modification
|
Date de publication |
2017
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication électronique |
2017-01-31
|
Volume |
57
|
Page/Identifiant |
1 - 29
|
Domaine(s) |
|
Mots-clés |
en
Diagnostic methods, Genome-wide map, Hybrid strains, Molecular markers, Sympatric species
|
DOI | 10.1016/j.mam.2016.11.012 |
Pubmed Id | 28159546 |
Origine :
Publication financée par une institution
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