Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease
Tatjana Ruskovska
(1)
,
Marika Massaro
(2)
,
Maria Annunziata Carluccio
(2)
,
Anna Arola-Arnal
(3)
,
Begoña Muguerza
(3)
,
Wim Vanden Berghe
(4)
,
Ken Declerck
(4)
,
Francisca Isabel Bravo
(3)
,
Nadia Calabriso
(2)
,
Emilie Combet
(5)
,
Eileen Gibney
(6)
,
Andreia Gomes
(7, 8)
,
Marie-Paule Gonthier
(9)
,
Elena Kistanova
(10)
,
Irena Krga
(11)
,
Pedro Mena
(12)
,
Christine Morand
(13)
,
Claudia Nunes dos Santos
(7, 14, 15)
,
Sonia de Pascual-Teresa
(16)
,
Ana Rodriguez-Mateos
(17)
,
Egeria Scoditti
(18)
,
Manuel Suárez
(3)
,
Dragan Milenkovic
(13)
1
UGD -
University Goce Delcev
2 IFC - Institute of Clinical Physiology
3 Universitat Rovira i Virgili
4 Universiteit Antwerpen = University of Antwerpen [Antwerpen]
5 University of Glasgow
6 UCD - University College Dublin [Dublin]
7 IBET - Instituto de Biologia Experimental e Tecnológica
8 ITQB - Instituto de Tecnologia Química e Biológica António Xavier
9 DéTROI - Diabète athérothrombose et thérapies Réunion Océan Indien
10 BAS - Bulgarian Academy of Sciences
11 University of Belgrade [Belgrade]
12 Department of Food Science, Human Nutrition Unit, The Phi² Laboratory of Phytochemicals in Physiology - LS9 Bioactives and Health, Interlab Group
13 UNH - Unité de Nutrition Humaine
14 NOVA - Universidade Nova de Lisboa = NOVA University Lisbon
15 CEDOC - Centro de Estudos de Doenças Crónicas
16 ICTAN-CSIC - Institute of Food Science, Technology and Nutrition
17 King‘s College London
18 Natl Res Council CNR, Inst Biol & Agr Biotechnol IBBA, Res Unit Pisa, Pisa, Italy
2 IFC - Institute of Clinical Physiology
3 Universitat Rovira i Virgili
4 Universiteit Antwerpen = University of Antwerpen [Antwerpen]
5 University of Glasgow
6 UCD - University College Dublin [Dublin]
7 IBET - Instituto de Biologia Experimental e Tecnológica
8 ITQB - Instituto de Tecnologia Química e Biológica António Xavier
9 DéTROI - Diabète athérothrombose et thérapies Réunion Océan Indien
10 BAS - Bulgarian Academy of Sciences
11 University of Belgrade [Belgrade]
12 Department of Food Science, Human Nutrition Unit, The Phi² Laboratory of Phytochemicals in Physiology - LS9 Bioactives and Health, Interlab Group
13 UNH - Unité de Nutrition Humaine
14 NOVA - Universidade Nova de Lisboa = NOVA University Lisbon
15 CEDOC - Centro de Estudos de Doenças Crónicas
16 ICTAN-CSIC - Institute of Food Science, Technology and Nutrition
17 King‘s College London
18 Natl Res Council CNR, Inst Biol & Agr Biotechnol IBBA, Res Unit Pisa, Pisa, Italy
Tatjana Ruskovska
- Fonction : Auteur
- PersonId : 781899
- ORCID : 0000-0002-4300-4891
Marika Massaro
- Fonction : Auteur
- PersonId : 762902
- ORCID : 0000-0001-6124-5077
Anna Arola-Arnal
- Fonction : Auteur
- PersonId : 804370
- ORCID : 0000-0001-6529-1345
Francisca Isabel Bravo
- Fonction : Auteur
- PersonId : 804371
- ORCID : 0000-0002-6468-3088
Andreia Gomes
- Fonction : Auteur
- PersonId : 795241
- ORCID : 0000-0003-2605-2565
Marie-Paule Gonthier
- Fonction : Auteur
- PersonId : 1230758
- ORCID : 0000-0002-2389-409X
Elena Kistanova
- Fonction : Auteur
- PersonId : 804372
- ORCID : 0000-0002-5239-1715
Irena Krga
- Fonction : Auteur
- PersonId : 775884
- ORCID : 0000-0002-2073-2896
Pedro Mena
- Fonction : Auteur
- PersonId : 784054
- ORCID : 0000-0003-2150-2977
Christine Morand
- Fonction : Auteur
- PersonId : 745929
- IdHAL : christine-morand
- ORCID : 0000-0001-8128-1032
- IdRef : 153776919
Egeria Scoditti
- Fonction : Auteur
- PersonId : 804373
- ORCID : 0000-0003-2753-8487
Manuel Suárez
- Fonction : Auteur
- PersonId : 757319
- ORCID : 0000-0003-0122-8253
Dragan Milenkovic
- Fonction : Auteur
- PersonId : 736748
- IdHAL : dragan-milenkovic
Résumé
Flavanol intake positively influences several cardiometabolic risk factors in humans. However, the specific molecular mechanisms of action of flavanols, in terms of gene regulation, in the cell types relevant to cardiometabolic disease have never been systematically addressed. On this basis, we conducted a systematic literature review and a comprehensive bioinformatic analysis of genes whose expression is affected by flavanols in cells defining cardiometabolic health: hepatocytes, adipocytes, endothelial cells, smooth muscle cells and immune cells. A systematic literature search was performed using the following pre-defined criteria: treatment with pure compounds and metabolites (no extracts) at low concentrations that are close to their plasma concentrations. Differentially expressed genes were analyzed using bioinformatics tools to identify gene ontologies, networks, cellular pathways and interactions, as well as transcriptional and post-transcriptional regulators. The systematic literature search identified 54 differentially expressed genes at the mRNA level inin vitromodels of cardiometabolic disease exposed to flavanols and their metabolites. Global bioinformatic analysis revealed that these genes are predominantly involved in inflammation, leukocyte adhesion and transendothelial migration, and lipid metabolism. We observed that, although the investigated cells responded differentially to flavanol exposure, the involvement of anti-inflammatory responses is a common mechanism of flavanol action. We also identified potential transcriptional regulators of gene expression: transcriptional factors, such as GATA2, NFKB1, FOXC1 or PPARG, and post-transcriptional regulators: miRNAs, such as mir-335-5p, let-7b-5p, mir-26b-5p or mir-16-5p. In parallel, we analyzed the nutrigenomic effects of flavanols in intestinal cells and demonstrated their predominant involvement in the metabolism of circulating lipoproteins. In conclusion, the results of this systematic analysis of the nutrigenomic effects of flavanols provide a more comprehensive picture of their molecular mechanisms of action and will support the future setup of genetic studies to pave the way for individualized dietary recommendations.
Domaines
Biochimie, Biologie Moléculaire| Format du dépôt | Fichier |
|---|---|
| Type de dépôt | Article dans une revue |
| Titre |
en
Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease
|
| Résumé |
en
Flavanol intake positively influences several cardiometabolic risk factors in humans. However, the specific molecular mechanisms of action of flavanols, in terms of gene regulation, in the cell types relevant to cardiometabolic disease have never been systematically addressed. On this basis, we conducted a systematic literature review and a comprehensive bioinformatic analysis of genes whose expression is affected by flavanols in cells defining cardiometabolic health: hepatocytes, adipocytes, endothelial cells, smooth muscle cells and immune cells. A systematic literature search was performed using the following pre-defined criteria: treatment with pure compounds and metabolites (no extracts) at low concentrations that are close to their plasma concentrations. Differentially expressed genes were analyzed using bioinformatics tools to identify gene ontologies, networks, cellular pathways and interactions, as well as transcriptional and post-transcriptional regulators. The systematic literature search identified 54 differentially expressed genes at the mRNA level inin vitromodels of cardiometabolic disease exposed to flavanols and their metabolites. Global bioinformatic analysis revealed that these genes are predominantly involved in inflammation, leukocyte adhesion and transendothelial migration, and lipid metabolism. We observed that, although the investigated cells responded differentially to flavanol exposure, the involvement of anti-inflammatory responses is a common mechanism of flavanol action. We also identified potential transcriptional regulators of gene expression: transcriptional factors, such as GATA2, NFKB1, FOXC1 or PPARG, and post-transcriptional regulators: miRNAs, such as mir-335-5p, let-7b-5p, mir-26b-5p or mir-16-5p. In parallel, we analyzed the nutrigenomic effects of flavanols in intestinal cells and demonstrated their predominant involvement in the metabolism of circulating lipoproteins. In conclusion, the results of this systematic analysis of the nutrigenomic effects of flavanols provide a more comprehensive picture of their molecular mechanisms of action and will support the future setup of genetic studies to pave the way for individualized dietary recommendations.
|
| Auteur(s) |
Tatjana Ruskovska
1
, Marika Massaro
2
, Maria Annunziata Carluccio
2
, Anna Arola-Arnal
3
, Begoña Muguerza
3
, Wim Vanden Berghe
4
, Ken Declerck
4
, Francisca Isabel Bravo
3
, Nadia Calabriso
2
, Emilie Combet
5
, Eileen Gibney
6
, Andreia Gomes
7, 8
, Marie-Paule Gonthier
9
, Elena Kistanova
10
, Irena Krga
11
, Pedro Mena
12
, Christine Morand
13
, Claudia Nunes dos Santos
7, 14, 15
, Sonia de Pascual-Teresa
16
, Ana Rodriguez-Mateos
17
, Egeria Scoditti
18
, Manuel Suárez
3
, Dragan Milenkovic
13
1
UGD -
University Goce Delcev
( 492308 )
- Macédoine du Nord
2
IFC -
Institute of Clinical Physiology
( 336271 )
- c/o Area di Ricerca "S. Cataldo"
Via Giuseppe Moruzzi, 1 - 56124 Pisa (PI)
- Italie
3
Universitat Rovira i Virgili
( 165020 )
- 43007 Tarragona
- Espagne
4
Universiteit Antwerpen = University of Antwerpen [Antwerpen]
( 147977 )
- Prinsstraat 13, 2000 Antwerpen
- Belgique
5
University of Glasgow
( 25887 )
- Glasgow G12 8QQ Scotland
- Royaume-Uni
6
UCD -
University College Dublin [Dublin]
( 83934 )
- Belfield, Dublin 4
- Irlande
7
IBET -
Instituto de Biologia Experimental e Tecnológica
( 345590 )
- Av. República
Qta. do Marquês
2780-157 Oeiras
- Portugal
8
ITQB -
Instituto de Tecnologia Química e Biológica António Xavier
( 140112 )
- Avenida da República
Estação Agronómica Nacional
2780-157 Oeiras
- Portugal
9
DéTROI -
Diabète athérothrombose et thérapies Réunion Océan Indien
( 418881 )
- Faculté des Sciences et techniques – Université de La Réunion 15 avenue René Cassin CS92003 97744 SAINT DENIS CEDEX 9
- La Réunion
10
BAS -
Bulgarian Academy of Sciences
( 302199 )
- 15th November #1 str, Sofia, 1040
- Bulgarie
11
University of Belgrade [Belgrade]
( 167343 )
- Studentski Trg 1, 11000 Belgrade
- Serbie
12
Department of Food Science, Human Nutrition Unit, The Phi² Laboratory of Phytochemicals in Physiology - LS9 Bioactives and Health, Interlab Group
( 538908 )
- Italie
13
UNH -
Unité de Nutrition Humaine
( 1002386 )
- Site INRAE Theix : 63122 Saint-Gènes-Champanelle // Site Clermont : UFR de Médecine et de Pharmacie, TSA 50400, 28 Place Henri Dunant, 63001 Clermont-Ferrand
- France
14
NOVA -
Universidade Nova de Lisboa = NOVA University Lisbon
( 327246 )
- Campus de Campolide
1099-085 Lisboa
- Portugal
15
CEDOC -
Centro de Estudos de Doenças Crónicas
( 204886 )
- Rua Camara Pestana, 6,1169-056 Lisboa
- Portugal
16
ICTAN-CSIC -
Institute of Food Science, Technology and Nutrition
( 538252 )
- Espagne
17
King‘s College London
( 327716 )
- Strand Campus, London WC2R 2LS
- Royaume-Uni
18
Natl Res Council CNR, Inst Biol & Agr Biotechnol IBBA, Res Unit Pisa, Pisa, Italy
( 420777 )
- Res Unit Pisa, Pisa, Italy
- Italie
|
| Langue du document |
Anglais
|
| Nom de la revue |
|
| Volume |
11
|
| Numéro |
6
|
| Page/Identifiant |
5040-5064
|
| Date de publication |
2020
|
| Audience |
Internationale
|
| Vulgarisation |
Non
|
| Comité de lecture |
Oui
|
| Licence |
Paternité
|
| Public visé |
Scientifique
|
| Domaine(s) |
|
| Financement |
|
| Mots-clés |
en
flavanol
|
| DOI | 10.1039/d0fo00701c |
| Pubmed Id | 32537624 |
| UT key WOS | 000542844600016 |
Origine :
Fichiers produits par l'(les) auteur(s)
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