Plasmodium infection is associated with cross‑reactive antibodies to carbohydrate epitopes on the SARS‑CoV‑2 Spike protein
Sarah Lapidus
(1)
,
Feimei Liu
(2)
,
Arnau Casanovas-Massana
(1)
,
Yile Dai
(2)
,
John D. Huck
(2)
,
Carolina Lucas
(2)
,
Jon Klein
(2)
,
Renata B. Filler
(2)
,
Madison S. Strine
(2)
,
Mouhamad Sy
(3)
,
Awa B. Deme
(3)
,
Aida S. Badiane
(3)
,
Baba Dieye
(3)
,
Ibrahima Mbaye Ndiaye
(3)
,
Younous Diedhiou
(3)
,
Amadou Moctar Mbaye
(3)
,
Cheikh Tidiane Diagne
(4)
,
Inès Vigan‑womas
(5)
,
Alassane Mbengue
(5)
,
Bacary D. Sadio
(6)
,
Moussa M. Diagne
(6)
,
Adam J. Moore
(1)
,
Khadidiatou Mangou
(5)
,
Fatoumata Diallo
(5)
,
Seynabou D. Sene
(5)
,
Mariama N. Pouye
(5)
,
Rokhaya Faye
(5)
,
Babacar Diouf
(5)
,
Nivison Jr Nery
(7, 8)
,
Federico Costa
(1, 7, 8)
,
Mitermayer Reis
(1, 7, 8)
,
M. Catherine Muenker
(1)
,
Daniel Z. Hodson
(1)
,
Yannick Mbarga
(9)
,
Ben Z. Katz
(10, 11)
,
Jason R. Andrews
(12)
,
Melissa Campbell
(2)
,
Ariktha Srivathsan
(1)
,
Kathy Kamath
(13)
,
Elisabeth Baum-Jones
(13)
,
Ousmane Faye
(6)
,
Amadou Alpha Sall
(6)
,
Juan Carlos Quintero Vélez
(14)
,
Michael Cappello
(2)
,
Michael Wilson
(15)
,
Choukri Ben-Mamoun
(2)
,
Fabrice A. Somé
(16)
,
Roch K. Dabiré
(16)
,
Carole Else Eboumbou Moukoko
(17, 18)
,
Jean Bosco Ouédraogo
(16)
,
Yap Boum
(19, 20)
,
John Shon
(13)
,
Daouda Ndiaye
(3)
,
Adam Wisnewski
(2)
,
Sunil Parikh
(1)
,
Akiko Iwasaki
(2)
,
Craig B. Wilen
(2)
,
Albert I. Ko
(1, 8)
,
Aaron M. Ring
(2)
,
Amy K. Bei
(1, 3, 5)
1
YSPH -
Yale School of Public Health
2 YSM - Yale School of Medicine [New Haven, Connecticut]
3 UCAD - Université Cheikh Anta Diop de Dakar [Sénégal]
4 Diatropix [Dakar, Sénégal]
5 Pôle Immunophysiopathologie et Maladies Infectieuses
6 Département de virologie - Virology Department [Dakar]
7 UFBA - Universidade Federal da Bahia = Federal University of Bahia
8 IGM - Instituto Gonçalo Moniz / Gonçalo Moniz Research Centre - Fiocruz Bahia [Salvador, Brésil]
9 Douala Military Hospital
10 [Northwestern University Medical School] - Feinberg School of Medicine [Northwestern University, Evanston]
11 Ann & Robert H. Lurie Children's Hospital of Chicago
12 Stanford University
13 Serimmune [Goleta, CA]
14 Universidad de Antioquia = University of Antioquia [Medellín, Colombia]
15 University of Ghana
16 Institut de Recherche en Sciences de la Santé (IRSS) / Centre Muraz
17 Université de Douala
18 Centre Pasteur du Cameroun
19 University of Yaoundé [Cameroun]
20 Epicentre [Paris] [Médecins Sans Frontières]
2 YSM - Yale School of Medicine [New Haven, Connecticut]
3 UCAD - Université Cheikh Anta Diop de Dakar [Sénégal]
4 Diatropix [Dakar, Sénégal]
5 Pôle Immunophysiopathologie et Maladies Infectieuses
6 Département de virologie - Virology Department [Dakar]
7 UFBA - Universidade Federal da Bahia = Federal University of Bahia
8 IGM - Instituto Gonçalo Moniz / Gonçalo Moniz Research Centre - Fiocruz Bahia [Salvador, Brésil]
9 Douala Military Hospital
10 [Northwestern University Medical School] - Feinberg School of Medicine [Northwestern University, Evanston]
11 Ann & Robert H. Lurie Children's Hospital of Chicago
12 Stanford University
13 Serimmune [Goleta, CA]
14 Universidad de Antioquia = University of Antioquia [Medellín, Colombia]
15 University of Ghana
16 Institut de Recherche en Sciences de la Santé (IRSS) / Centre Muraz
17 Université de Douala
18 Centre Pasteur du Cameroun
19 University of Yaoundé [Cameroun]
20 Epicentre [Paris] [Médecins Sans Frontières]
Feimei Liu
- Fonction : Auteur
- PersonId : 819163
- ORCID : 0000-0003-2663-6544
Arnau Casanovas-Massana
- Fonction : Auteur
- PersonId : 806365
- ORCID : 0000-0002-3301-6143
John D. Huck
- Fonction : Auteur
- PersonId : 819839
- ORCID : 0000-0002-0755-3850
Awa B. Deme
- Fonction : Auteur
- PersonId : 819840
- ORCID : 0000-0002-3119-6419
Aida S. Badiane
- Fonction : Auteur
- PersonId : 799571
- ORCID : 0000-0002-1118-4998
Cheikh Tidiane Diagne
- Fonction : Auteur
- PersonId : 794492
- ORCID : 0000-0003-2119-9349
- IdRef : 177276819
Inès Vigan‑womas
- Fonction : Auteur
- PersonId : 736886
- IdHAL : ines-vigan-womas
- ORCID : 0000-0001-7848-9297
- IdRef : 241942020
Alassane Mbengue
- Fonction : Auteur
- PersonId : 799357
- ORCID : 0000-0002-4210-6167
- IdRef : 164805206
Jason R. Andrews
- Fonction : Auteur
- PersonId : 819841
- ORCID : 0000-0002-5967-251X
Akiko Iwasaki
- Fonction : Auteur
- PersonId : 766959
- ORCID : 0000-0002-7824-9856
Craig B. Wilen
- Fonction : Auteur
- PersonId : 819843
- ORCID : 0000-0003-2495-9403
Albert I. Ko
- Fonction : Auteur
- PersonId : 802512
- ORCID : 0000-0001-9023-2339
Aaron M. Ring
- Fonction : Auteur
- PersonId : 819844
- ORCID : 0000-0003-3699-2446
Amy K. Bei
Connectez-vous pour contacter l'auteur
- Fonction : Auteur correspondant
- PersonId : 1140243
Connectez-vous pour contacter l'auteur
Résumé
Sero-surveillance can monitor and project disease burden and risk. However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool. False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries. Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity. Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity. Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays. ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein. The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro. Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity. Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.
Domaines
Microbiologie et Parasitologie Biologie moléculaire Biologie cellulaire Immunologie Santé publique et épidémiologieFormat du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Plasmodium infection is associated with cross‑reactive antibodies to carbohydrate epitopes on the SARS‑CoV‑2 Spike protein
|
Résumé |
fr
Sero-surveillance can monitor and project disease burden and risk. However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool. False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries. Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity. Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity. Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays. ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein. The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro. Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity. Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.
|
Auteur(s) |
Sarah Lapidus
1
, Feimei Liu
2
, Arnau Casanovas-Massana
1
, Yile Dai
2
, John D. Huck
2
, Carolina Lucas
2
, Jon Klein
2
, Renata B. Filler
2
, Madison S. Strine
2
, Mouhamad Sy
3
, Awa B. Deme
3
, Aida S. Badiane
3
, Baba Dieye
3
, Ibrahima Mbaye Ndiaye
3
, Younous Diedhiou
3
, Amadou Moctar Mbaye
3
, Cheikh Tidiane Diagne
4
, Inès Vigan‑womas
5
, Alassane Mbengue
5
, Bacary D. Sadio
6
, Moussa M. Diagne
6
, Adam J. Moore
1
, Khadidiatou Mangou
5
, Fatoumata Diallo
5
, Seynabou D. Sene
5
, Mariama N. Pouye
5
, Rokhaya Faye
5
, Babacar Diouf
5
, Nivison Jr Nery
7, 8
, Federico Costa
1, 7, 8
, Mitermayer Reis
1, 7, 8
, M. Catherine Muenker
1
, Daniel Z. Hodson
1
, Yannick Mbarga
9
, Ben Z. Katz
10, 11
, Jason R. Andrews
12
, Melissa Campbell
2
, Ariktha Srivathsan
1
, Kathy Kamath
13
, Elisabeth Baum-Jones
13
, Ousmane Faye
6
, Amadou Alpha Sall
6
, Juan Carlos Quintero Vélez
14
, Michael Cappello
2
, Michael Wilson
15
, Choukri Ben-Mamoun
2
, Fabrice A. Somé
16
, Roch K. Dabiré
16
, Carole Else Eboumbou Moukoko
17, 18
, Jean Bosco Ouédraogo
16
, Yap Boum
19, 20
, John Shon
13
, Daouda Ndiaye
3
, Adam Wisnewski
2
, Sunil Parikh
1
, Akiko Iwasaki
2
, Craig B. Wilen
2
, Albert I. Ko
1, 8
, Aaron M. Ring
2
, Amy K. Bei
1, 3, 5
1
YSPH -
Yale School of Public Health
( 360631 )
- 60 College Street P.O. Box 208034 New Haven
- États-Unis
2
YSM -
Yale School of Medicine [New Haven, Connecticut]
( 51214 )
- Yale University School of Medicine - 333 Cedar Street - New Haven, CT 06510
- États-Unis
3
UCAD -
Université Cheikh Anta Diop de Dakar [Sénégal]
( 1175215 )
- Université Cheikh Anta Diop de Dakar - Rectorat, BP 5005 Dakar-fann
- Sénégal
4
Diatropix [Dakar, Sénégal]
( 1084391 )
- 36 Avenue Pasteur, B.P. 220, Dakar
- Sénégal
5
Pôle Immunophysiopathologie et Maladies Infectieuses
( 1084392 )
- 36 Avenue Pasteur, B.P. 220, Dakar
- Sénégal
6
Département de virologie - Virology Department [Dakar]
( 1075020 )
- 36, Avenue Pasteur, B.P. 220 - Dakar
- Sénégal
7
UFBA -
Universidade Federal da Bahia = Federal University of Bahia
( 303563 )
- Rua Augusto Viana, s/n - Palácio da Reitoria, Canela.
40.110-909, Salvador
- Brésil
8
IGM -
Instituto Gonçalo Moniz / Gonçalo Moniz Research Centre - Fiocruz Bahia [Salvador, Brésil]
( 107012 )
- Rua Waldemar Falcão 121 - Candeal, Salvador, BA
- Brésil
9
Douala Military Hospital
( 1084400 )
- Douala
- Cameroun
10
[Northwestern University Medical School] -
Feinberg School of Medicine [Northwestern University, Evanston]
( 360635 )
- Arthur J. Rubloff Building – 420 East Superior Street – Chicago, IL 60611
- États-Unis
11
Ann & Robert H. Lurie Children's Hospital of Chicago
( 418183 )
- Chicago
Illinois
- États-Unis
12
Stanford University
( 73500 )
- 450 Serra Mall, Stanford, CA 94305-2004
- États-Unis
13
Serimmune [Goleta, CA]
( 1084399 )
- 150 Castilian Drive, Suite 100, Goleta, CA 93117
- États-Unis
14
Universidad de Antioquia = University of Antioquia [Medellín, Colombia]
( 118314 )
- Calle 67 Número 53 - 108 Medellín
- Colombie
15
University of Ghana
( 230533 )
- P.O. Box LG 25
Legon, Accra
- Ghana
16
Institut de Recherche en Sciences de la Santé (IRSS) / Centre Muraz
( 350516 )
- Bobo-Dioulasso
- Burkina Faso
17
Université de Douala
( 217369 )
- B.P. 2701 Douala
- Cameroun
18
Centre Pasteur du Cameroun
( 55917 )
- B.P. 1274 Yaoundé
- Cameroun
19
University of Yaoundé [Cameroun]
( 303594 )
- Cameroun
20
Epicentre [Paris] [Médecins Sans Frontières]
( 335654 )
- 55 Rue Crozatier, 75012 Paris
- France
|
Audience |
Internationale
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Langue du document |
Anglais
|
Licence |
Paternité - Pas d'utilisation commerciale - Pas de modification
|
Nom de la revue |
|
Date de publication |
2022
|
Domaine(s) |
|
Financement |
|
Données associées | |
Mots-clés |
en
Infectious-disease diagnostics, Malaria, Viral infection, COVID-19
|
DOI | 10.1038/s41598-022-26709-7 |
Pubmed Id | 36550362 |
PubMed Central | PMC9778468 |
Origine :
Publication financée par une institution
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