Sub-micrometric spatial distribution of amorphous and crystalline carbonates in biogenic crystals using coherent Raman microscopy
Hamadou Dicko
(1)
,
Tilman Grünewald
(1)
,
Patrick Ferrand
(1)
,
Jérémie Vidal-Dupiol
(2)
,
Vaihiti Teaniniuraitemoana
(3)
,
Manaari Sham Koua
(3)
,
Gilles Le Moullac
(3)
,
Jérémy Le Luyer
(3)
,
Denis Saulnier
(3)
,
Virginie Chamard
(1)
,
Julien Duboisset
(4)
Tilman Grünewald
- Fonction : Auteur
- PersonId : 754330
- IdHAL : tilman-grunewald
- ORCID : 0000-0002-5621-605X
Patrick Ferrand
- Fonction : Auteur
- PersonId : 1171
- IdHAL : patrick-ferrand
- ORCID : 0000-0002-7395-5663
- IdRef : 109970888
Jérémie Vidal-Dupiol
- Fonction : Auteur
- PersonId : 747176
- IdHAL : jeremie-vidal-dupiol
- ORCID : 0000-0002-0577-2953
- IdRef : 199462380
Virginie Chamard
- Fonction : Auteur
- PersonId : 6769
- IdHAL : virginie-chamard
- ORCID : 0000-0002-6894-4169
- IdRef : 159044820
Résumé
In living organisms, calcium carbonate biomineralization combines complex bio-controlled physical and chemical processes to produce crystalline hierarchical hard tissues (usually calcite or aragonite) typically from an amorphous precursor phase. Understanding the nature of the successive transient amorphous phases potentially involved in the amorphous-to-crystalline transition requires characterization tools, which are able to provide a spatial and spectroscopic analysis of the biomineral structure. In this work, we present a highly sensitive coherent Raman microscopy approach, which allows one to image molecular bond concentrations in post mortem shells and living animals, by exploiting the vibrational signature of the different carbonates compounds. To this end, we target the nu_1 calcium carbonate vibration mode and produce spatially and spectroscopically resolved images of the shell border of a mollusk shell, the Pinctada margaritifera pearl oyster. A novel approach is further presented to efficiently compare the amount of amorphous carbonate with respect to its crystalline counterpart. Finally, the whole microscopy method is used to image in vivo the shell border and demonstrate the feasibility and the reproducibility of the technique. These findings open chemical imaging perspectives for the study of biogenic and bio-inspired crystals.
Domaines
Matière Condensée [cond-mat]Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Sub-micrometric spatial distribution of amorphous and crystalline carbonates in biogenic crystals using coherent Raman microscopy
|
Résumé |
en
In living organisms, calcium carbonate biomineralization combines complex bio-controlled physical and chemical processes to produce crystalline hierarchical hard tissues (usually calcite or aragonite) typically from an amorphous precursor phase. Understanding the nature of the successive transient amorphous phases potentially involved in the amorphous-to-crystalline transition requires characterization tools, which are able to provide a spatial and spectroscopic analysis of the biomineral structure. In this work, we present a highly sensitive coherent Raman microscopy approach, which allows one to image molecular bond concentrations in post mortem shells and living animals, by exploiting the vibrational signature of the different carbonates compounds. To this end, we target the nu_1 calcium carbonate vibration mode and produce spatially and spectroscopically resolved images of the shell border of a mollusk shell, the Pinctada margaritifera pearl oyster. A novel approach is further presented to efficiently compare the amount of amorphous carbonate with respect to its crystalline counterpart. Finally, the whole microscopy method is used to image in vivo the shell border and demonstrate the feasibility and the reproducibility of the technique. These findings open chemical imaging perspectives for the study of biogenic and bio-inspired crystals.
|
Auteur(s) |
Hamadou Dicko
1
, Tilman Grünewald
1
, Patrick Ferrand
1
, Jérémie Vidal-Dupiol
2
, Vaihiti Teaniniuraitemoana
3
, Manaari Sham Koua
3
, Gilles Le Moullac
3
, Jérémy Le Luyer
3
, Denis Saulnier
3
, Virginie Chamard
1
, Julien Duboisset
4
1
COMiX -
Coherent Optical Microscopy and X-rays
( 445088 )
- France
2
IHPE -
Interactions Hôtes-Pathogènes-Environnements
( 1100741 )
- Université de Perpignan Via Domitia Bâtiment R 52 Avenue Paul Alduy 66860 Perpignan cedex (Languedoc-Roussillon)
- France
3
LabEX CORAIL -
Laboratoire d'Excellence CORAIL
( 209113 )
- 58 avenue Paul Alduy 66860 Perpignan CEDEX - Tél. : 04 30 19 23 32 - contact@labex-corail.fr
- France
4
MOSAIC -
MOSAIC
( 389416 )
- France
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2022-12
|
Date de publication électronique |
2022-10-27
|
Volume |
214
|
Numéro |
4
|
Page/Identifiant |
107909
|
Domaine(s) |
|
Projet(s) Européen(s) |
|
DOI | 10.1016/j.jsb.2022.107909 |
Origine :
Fichiers produits par l'(les) auteur(s)
Loading...